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Johns Hopkins University School of Medicine
Department of Cell Biology
725 N. Wolfe St., 114 WBSB
Baltimore, MD 21205

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Protocols


Ammonium Sulfate Binding	GTPgS Activation of ACA
CHIFF Preparation	In vivo Actin Polymerization
Purification of cAR1 from CHIFFs	In vitro Actin Polymerization
MolBioProtocols	Fluorimeter Start and Shut Down
Western Blot of cAR1	FTPSWAP
Photo affinity Labeling of cAR1	Spot Screening of Phenotypes
Preparation of Cells with Upper Band and Lower Band	Electroporation
GTP Inhibition of Binding	Phagocytosis
Antibody Staining for Gold EM	Needle Assay for Chemotaxis
Bead Binding Assay	Screen of Colonies
Equilibrium Dialysis Binding	Random Mutagenesis
In vivo Activation of ACA	REMI
Crac Supernatant Preparation
In Vivo Translocation of CRAC

Recipes for Media and Stock Solutions

Rex Chisholm's Protocols

This page was last edited 07/03/2003